Latest Posts
News, insights, and updates from RootPath.
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How do we ensure all MHCs of interest are expressed?
In cell-based assays of TCR:pMHC recognition, such as our TCR Decoder™ service, it is critical that all functional candidate MHC molecules are expressed. This is easier said than done. Here’s how we ensure this through an elaborate scheme, how biology and common reagents throw curve balls at us, and how we overcome the challenges.
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How we ensure antigen expression
In cell-based assays of TCR:pMHC recognition, such as our TCR Decoder™ service, it is critical that all functional candidate MHC molecules are expressed. This is easier said than done. Here’s how we ensure this through an elaborate scheme, how biology and common reagents throw curve balls at us, and how we overcome the challenges.
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Bringing TCR:pMHC Validation to the Digital Age
With the advances of single-cell sequencing technologies and AI-based protein design, discovery of candidate TCR (and TCR-mimicking binder) sequences has never been faster. However, experimental methods for TCR:pMHC validation appear to be stuck in the 20th century. Existing tools such as soluble pMHC multimers, B-lymphoblastoid cell lines (B-LCLs) and monocyte-derived dendritic cells (MDDCs) are often unavailable, while preparation of TCR-T cells is also slow and low-throughput. In this post, I discuss how services like RootPath’s TCR Decoder™ addresses all of these issues, and allow you to turn digital information such as TCR sequences, patient HLA types and candidate antigen sequences, into cell-based functional readout in as short as 3 to 4 weeks, dozens to hundreds of TCR sequences at a time.
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Pool or Array? Which one should I use?
While our claim to fame, so to speak, is the Nature Biotechnology paper describing the pool synthesis technology.
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Combinatorial Mutagenesis -- maximize your sequence search space
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Welcome to the RootPath Blog
We’re launching the RootPath blog to share updates on our gene synthesis platform, protein engineering services, and the science behind what we build.
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